Figure 1.

Generation of a large RNAseq dataset of midbrain dopamine neurons. A) Schematic of single nucleus isolation pipeline using microfluidic chip-based sorting from n=8 mice. B) UMAP representation of clusters. Of note, putative clusters 16 and 21 are shown in grey, and are removed from analyses due to coexpression of glial markers. C) Expression of the defining midbrain dopaminergic neuron markers vesicular monoamine transporter 2 (Vmat2; Slc18a2), DOPA decarboxylase (Ddc), tyrosine hydroxylase (Th), and dopamine transporter (Dat; Slc6a3). Robust expression is observed throughout all clusters. D) Dot plot showing expression of several markers of previously described dopamine neuron subtypes, consistent with finding additional heterogeneity within established subtypes. E) Expression patterns of midbrain floorplate markers. All clusters show robust expression with exception of 0 and 17, which indicate these populations are not midbrain DA neurons but might be located in nearby hypothalamic nuclei.