Figure 6. Immune Cell Adhesion Assay with ICAM-1 Knock-Out (KO) Pluripotent Stem Cell (PSC)-Derived Endothelial Cells (ECs).

H9 wild type (WT) and ICAM-1 KO PSCs were differentiated into high-purity CD31+CD144+ ECs and stimulated with TNFα (10ng/ml) and IFNγ (50ng/ml) for 48 hours. As in Figure 1, U937s were co-cultured with both cell types and washed. Images were acquired on ECHO Revolve | R4 microscope. **** p<0.0001, biological replicates=3. Statistical analysis was performed via Prism 10.2.2 software. ROI = Region of Interest