Figure 5. The Fc portion of all cocktail mAbs contributes to ADCC.

(A) HIV-1_CH058TF-infected primary CD4+ T cells were stained with a total of 5 μg/mL of indicated antibody combination in presence of CJF-III-288 depicted in blue 48h post-infection. Flow cytometry was performed to detect antibody binding using appropriated secondary antibody. The graph represents the MFI of Alexa-Fluor 647 obtained in at least 6 independent experiments. (B) HIV-1_CH58TF-infected primary CD4+ T cells were used as target cells, while autologous non-infected PBMCs were used as effector cells in our FACS-based ADCC assay in the presence of 5 μg/mL of indicated combination of nnAbs. The graph represents the percentage of ADCC obtained in presence of indicated combination of antibodies in at least 6 independent experiments. Statistical significance was tested using (A-B) Wilcoxon test or paired t-test according to normality. (*, P < 0.05; **, P < 0.01).