Figure 6. Binding and elimination of HIV-1 infected MDMs by ADCC.
(A) HIV-1AD8-infected primary MDMs were stained with a total of 5 μg/mL total of the combined antibodies in presence of CJF-III-288 depicted in blue or DMSO depicted in gray 5 days post-infection. Flow cytometry was performed to detect antibody binding. (B) The graph represents the MFI of Alexa-Fluor 647 obtained in at least 5 independent experiments. (C) HIV-1AD8-infected primary MDMs were used as target cells, while autologous non-infected PBMCs were used as effector cells in our FACS-based ADCC assay in the presence of 5 μg/mL of indicated combination of nnAbs. The graph represents the percentage of ADCC obtained in presence of indicated combination of antibodies in at least 5 independent experiments. (B-C) Wilcoxon test or paired t-test according to normality. p-values are plotted.