FIG. 2.

Steady-state immunoprecipitation analysis. PK15 cells were infected at an MOI of 10 with either PRV Be (wild type), PRV 91 (gE null), PRV 98 (gI null), PRV 107 (anc gE), PRV 26 (sec gE), PRV 108 (anc gI), PRV 109 (sec gI), PRV 110 (anc gI/anc gE), or PRV 111 (sec gI/sec gE) and labeled for 12 h with [³⁵S]methionine-[³⁵S]cysteine prior to preparing cell lysates. Immunoprecipitations were performed on extracts using either rabbit polyvalent antiserum to the immature form of gI (A), rabbit polyvalent antiserum to gE (B), monoclonal antiserum to gE when it is complexed with gI (C), or goat polyvalent antiserum to gC (D). Panels A, B, and D show immunoprecipitations performed with denatured extracts, while the extracts for panel C were not denatured prior to immunoprecipitation. Circles and squares in panel C denote gE- gI-specific bands, respectively. Positions of apparent molecular mass markers (kilodaltons) are shown on the left.