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. 2024 Jun 19;21:120. doi: 10.1186/s12954-024-01023-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Sensitivity analyses of SARS-CoV-2 seroprevalence. Note. The dot plots show levels of antibodies (log 10) to indicated SARS-CoV-2 proteins in sera from indicated cohorts. Each dot represents a different sample. Spike-FL: full-length spike protein from SARS-CoV-2. RBD : receptor-binding domain. Bead-based arrays with virus proteins were incubated with serum diluted 1:100, labelled with R-Phycoerythrin (R-PE)-conjugated anti-human IgG Fc and analyzed by flow cytometry. The values correspond to R-PE median fluorescence intensity (MFI) measured for beads with indicated virus protein divided by the MFI measured for beads with neutravidin only. The dashed lines correspond to cutoffs set by analyzing prepandemic samples (Fig. 2a)