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FACS analysis of cell surface Env expression. Cell surface immunostaining with an anti-gp41 monoclonal antibody (T32) was performed using CEM (12D-7) cells infected with wt or mutant NL4-3 virions pseudotyped with VSV-G. Averages of duplicate experiments are presented ± standard deviations. Solid bars represent mean fluorescence intensities determined by FACScan; open bars indicate percent Env-positive cells. Approximately 40% of cultures infected with the wt were Env positive.
