FIG. 7.

Analysis of wt and mutant Env incorporation into virions. (A) Radioimmunoprecipitation analysis of wt, d8, 34VI, and 34VI/d8. CEM (12D-7) cells were infected with wt or mutant virions pseudotyped with VSV-G. Labeling and immunoprecipitation were performed as indicated in the Fig. 3 legend. (B) Analysis of gp41 by Western blotting. Cell and virion lysates were prepared from CEM (12D-7) cells infected with VSV-G-pseudotyped KFS (Env-minus), NL4-3, d8, 34VI, or 34VI/d8. Samples were transferred to polyvinylidene difluoride membranes, blotted with an anti-gp41 monoclonal antibody (T32), and reprobed with AIDS patient sera to detect p24 (CA) (Materials and Methods). These results are representative of duplicate experiments.