TABLE 1.
MOI | Infection of myotubes (after differentiation)a:
|
Infection of cells prior to differentiationa:
|
||||
---|---|---|---|---|---|---|
2.5% FBSb | F10-based mediumb | SKGM-based mediumb | 2.5% FBS | F10-based medium | SKGM-based medium | |
1 × 104 | 200 | 600 | NDc | 300 | 600 | ND |
1 × 105 | 200 | 800 | 1900 | 310 | 1,000 | 1,500 |
5 × 105 | ND | 1,700 | ND | ND | 1,300 | ND |
Total expression of hFIX in nanograms per 106 cells per day.
The cells were maintained in either of the three culture media as described in the text and Fig. 2.
ND, not done.