Expression and ISO-induced phosphorylation of Ca2+ signaling targets in WT and PKD1 cKO mice. A. Phospholamban (PLB) expression and phosphorylation at Ser16. Data points represent individual hearts (WT, N = 6; KO, N = 6). B. Cardiac troponin I (TnI) expression and phosphorylation at Ser 22/23 (pTnI) in representative blots (a) and pooled data (b-c). Data points are from 8 WT and 7 KO, hearts. C, RyR2 expression and phosphorylation at S2814, S2808 (pRyR) in representative blots (a) and pooled data (b-d). Data are from 7 WT and 6 KO hearts. D, Total SERCA2 expression representative blots (a) and pooled data (b) from 8 WT and 7 KO hearts. Differences were considered statistically significant if P < 0.05. Experiments were performed in technical triplicates for each blot. Representative blots were cropped from original full blots (included in the Supplemental data).