Fig. 10. Synergistic otoprotection between zorifertinib and AZD5438 against excitotoxicity in zebrafish neuromasts.

Five-dpf zebrafish were preincubated with KA (500 μM) or regular fish water for 1 hour and then incubated for 2 hours with a combination of AZD5438 (CDK2 inhibitor) and zorifertinib (EGFR inhibitor) or with the individual compounds. Results are expressed as the number of hair cells per neuromast ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 versus KA alone (one-way ANOVA). A1, AZD5438 50 nM; A2, AZD5438 1 nM; A3, AZD5438 10 pM; Z1, zorifertinib 50 nM; Z2, zorifertinib 1 nM; Z3, zorifertinib 10 pM.