Fig. 2. EGFR signaling in the adult mammalian cochlea.

Single-cell RNA-seq reveals EGFR ligands, receptors, and downstream targets in hair and supporting cells. (A) Uniform Manifold Approximation and Projection plot with Leiden clustering showing supporting cell (blue), outer hair cell (orange), and inner hair cell (green) clusters in adult P28 C57BL6 mice. (B) Stacked violin plot showing hair and supporting cell markers in the clusters. (C and D) Expression levels and distribution of ligands (Egf and Tgfa), receptors (Egfr and Erbb2), and downstream targets (Akt1, Erk1/Mapk3, Map2k2, and Stat3) in the hair and supporting cell clusters. All single-cell RNA-seq analysis was done using Scanpy 1.9.3 (116). The code used is available at https://nbviewer.org/github/renevq/jupyter-notebooks/blob/main/Hang_data.ipynb. Similar results were obtained on the basis of our published dataset (48).