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. 2024 Jun 19;10(25):eadk2299. doi: 10.1126/sciadv.adk2299

Fig. 6. Conditional knockout of EGFR shows protection against NIHL.

Fig. 6.

(A) Baseline (BL) (pre-noise exposure) ABR thresholds of Pax2Cre; EGFRflox/flox mice and Pax2Cre; EGFR+/+ control littermates measured at 4 to 6 weeks of age. The mice were on a mixed background of CD1, CBA/CaJ, and C57BL/6 strains. No significant differences were detected between cKO and WT; n = 6 to 7. (B) Pax2Cre; EGFRflox/flox mice (n = 6) exhibited significantly smaller ABR threshold shifts [both 1 day (1D) for TTS and 14 days (14D) for PTS) than wild-type (WT) littermate controls (Pax2Cre; EGFR+/+; n = 6) C) ABR wave 1 amplitudes for 16 kHz stimuli respectively showed larger amplitudes at suprathreshold levels in the cKO compared to the WT but did not reach statistical significance. Two-way ANOVA, Holm-Šidák post hoc test; ****P < 0.0001 and ***P = 0.0007; black (1D) and magenta (14D). Data are presented as means ± SEM.