TABLE 1.
The effect of cysteine mutations in TVBS1 upon subgroup B and E viral entrya
| TVBS1(ΔDD) construct | Residue
|
Mean (SD) colonies/ml of virus
|
||||
|---|---|---|---|---|---|---|
| 46 | 59 | 62 | 77 | MLV-lacZ (EnvB) | MLV-lacZ (EnvE) | |
| WT | C | C | C | C | 2.2 × 105 (4.9 × 104) | 1.9 × 105 (3.6 × 104) |
| C46S | S | C | C | C | 6.3 × 105 (5.1 × 103) | 24.3 (5.1) |
| C46S/C62S | S | C | S | C | 5.3 × 105 (3.1 × 104) | 4.4 (2) |
| C59S | C | S | C | C | 4.9 × 105 (2.4 × 105) | 2.2 (2.7) |
| C59S/C77S | C | S | C | S | 2.3 × 105 (1.8 × 104) | 1.1 (1.9) |
| C62S/C77S | C | C | S | S | 4.0 × 105 (1.3 × 105) | 2.8 × 103 (8.9 × 102) |
| C77S | C | C | C | S | 1.6 × 105 (2.6 × 104) | 33 (58) |
| C46S/C62S/C77S | S | C | S | S | 8.7 × 104 (3.5 × 103) | 0 |
| C46S/C59S/C62S/C77S | S | S | S | S | 1.1 × 105 (2.1 × 104) | 0 |
a
Wild-type (WT) or mutant forms of TVBS1(ΔDD) were tested for their ability to mediate entry of MLV-LacZ (EnvB) and MLV-LacZ (EnvE). The averages from three independent experiments are shown with standard deviations in parentheses. C, cysteine; S, serine.