Figure 5. mIDA induced transcription of defense-associated marker genes.
(a) Transcripts of FRK1, MYB51, and PEP3 in WT Col-0 seedlings exposed to 1 µM mIDA for 1 hr (red) and to 1 µM mIDA for 12 hr (blue) compared to untreated tissue (black). RNA levels were measured by RT-qPCR analysis. ACTIN was used to normalize mRNA levels. The figure represents three biological replicates with four technical replicates. Statistical analyses was performed comparing induction times of individual genes using one-way ANOVA and post-hoc Tukey’s test (p<0.05). (b) pMYB51:YFPN expression is enhanced in roots after 7 hr exposure to 1 μM mIDA peptide compared to untreated roots (control), scale bar = 50 μm, maximum intensity projections of z-stacks, magenta = propidium iodide stain.
Figure 5—figure supplement 1. mIDA induced enhanced transcription of defense-associated marker genes is only partially dependent on the HAE and HSL2 receptors.
RT-qPCR data showing transcription of FRK1, MYB51, and PEP3 in Col-0 WT and hae hsl2 seedlings exposed to 1 µM mIDA for 1 hr compared to untreated seedlings (No treatment). ACTIN was used to normalize mRNA levels. Figure represents three biological replicates with four technical replicates. Statistical analyses was performed comparing samples within same gene, using two-way ANOVA and post-hoc Tukey’s test (p<0.05).
Figure 5—figure supplement 2. mIDA and flg22 induced expression of pMYB51:YFP.
Representative microscopic pictures of 7-days-old pMYB51:YFPN roots from seedlings transferred to liquid medium with 1 μM flg22 or 1 μM mIDA for 1, 3, 5, and 7 hr using a fluorescent microscope. Control seedlings were transferred to medium with no peptide and imaged in the same time frame. Fluorescent nuclei could be observed in a similar temporal pattern in roots subjected to mIDA and flg22. Scale bar = 100 μm, single plane images using a Zeiss Axioplan2 microscope with an AxioCam HRc, 20 x air objective and a YFP filter (Excitation: 500/20, Beamsplitter: FT515, Emission: 535/50), no imaging processing was performed, t=time in h. n=3, experiment repeated three times.