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. 2024 Apr 11;13:e85964. doi: 10.7554/eLife.85964

Figure 7. Statins supress proinflammatory cytokines and enhance anti-inflammatory factors in lipopolysaccharide (LPS)- or IL-4-activated macrophages.

Figure 7.

(A) RAW 264.7 macrophages treated with statins (lovastatin, 7 + 200 µM mevalonate; 2 days) or without were stimulated with LPS (100 ng/ml) for 3 hr. (a) Heatmap of differentially expressed genes by statins. (b) Gene Ontology (GO) analysis of downregulated genes by statins. (c) GO analysis of upregulated genes by statin. (B) Bone marrow-derived macrophages (BMDMs) treated with or without compactin (10 + 200 µM mevalonate; 2 days) are stimulated by LPS (50 ng/ml, 3 hr). Gene expressions are analyzed by qPCR. (C) BMDMs treated with or without compactin are stimulated by IL-4 (20 ng/ml, 6 hr) and gene expressions are analyzed by qPCR. (D) Chromatin-immunoprecipitation (ChIP) analysis of H3K27me3 in BMDMs with or without compactin treatment. The inactive gene desert is used as input control. Data are representative of at least 2 independent experiments with 3 samples per group and data are presented as mean ± standrad deviation (SD). Statistical analysis was performed using unpaired, two-tailed Student’s t-test. Asterisks (*) and (**) indicate a significant difference with p < 0.05 and p < 0.001. A hashtag (#) indicates not significant.