Figure 9. In vivo statin feeding in mice reduces cholesterol and upregulates Jmjd3 in the peritoneal macrophages, which conveys anti-inflammatory phenotype.

(A) Total cholesterol contents (free and esterified) in freshly isolated mouse peritoneal macrophages from simvastatin-fed (100 μg/kg/day, 14 days) and control mice. (B) Jmjd3 gene expression in peritoneal macrophages from simvastatin-fed (statin) and control mice. (C) Freshly isolated mouse peritoneal macrophages from simvastatin-fed and control mice are stimulated by lipopolysaccharide (LPS) (100 ng/ml, 6 hr). Gene expressions are analyzed by qPCR. (D) Freshly isolated mouse peritoneal macrophages from simvastatin-fed and control mice are stimulated by IL-4 (20 ng/ml, 6 hr) and gene expressions are analyzed by qPCR. Data are representative of 3 independent experiments with 3–4 samples per group and presented as mean ± standard deviation (SD). Statistical analysis was performed using unpaired, two-tailed Student’s t-test. Asterisks (*), (**), and (***) indicate a significant difference with p < 0.05, p < 0.005, and p < 0.001, respectively.