Extended Data Fig. 9. Relevance of neural classification in pediatric and adolescent patients diagnosed with H3K27-altered diffuse midline glioma (DMG).

a). Association of tumor location with neural signature. Two-tailed Student’s t-test. b). Volcano plot showing differentially methylated CpG sites of genes of the invasivity signature, neuronal signature, and trans-synaptic signaling signature. c). Cell state composition analysis in low- and high-neural DMG. d). Synaptic gene expression (PTPRS, ARHGEF2, GRIK2, DNM3, LRRTM2, GRIK5, NLGN4X, NRCAM, MAP2, INA, TMPRSS9)⁶ is significantly correlated with the stem cell-like state of DMG cells calculated by an overlap of single-cell DNA methylation and single-cell RNA sequencing (599 cells from 3 study participants) measurements. Simple linear regression. e – h). Kaplan–Meier survival analysis of 72 DMG patients under 18 years of age with a low- and high-neural DMG. Error bands representing the 95% confidence interval. Log-rank test, e) P = 0.0017, f) P = 0.0022, g) P = 0.0882, and h) P = 0.3236.