Extended Data Fig. 6. Cytokine driven or further effects of ACS plasma on immune cells.

(a) Monocyte phenotyping after incubation with sterile ACS (TP1-3M) or CCS (TP0M) plasma upon IL6 inhibition by flow cytometry. All MFIs were normalized to the respective CCS plasma average of the marker. Comparison between isotype and IL6 inhibition (paired dataset). Parametric data were analyzed using the multiple paired t-test (two-sided). *p≤0.05, **p≤0.01, ***p≤0.001. Illustration of paired data by connecting the data points. (b) T cell phenotyping after incubation with sterile ACS (TP1-4M) or CCS (TP0M) plasma by flow cytometry. Individual timepoints of sterile ACS plasma (randomized n=8) compared to CCS, mean value of at least 5 PBMC donors incubated with each patient plasma. Parametric distributed data were analyzed using the Ordinary One-Way ANOVA with correction for multiple comparisons by Dunnett’s test; non-parametric distributed data were analyzed using the Kruskal-Wallis test with correction for multiple comparisons by Dunn’s test. In case only the ordinary one-way ANOVA or Kruskal–Wallis test was significant, graphs are marked with a vertical bar on top. For the following comparisons, only the post hoc was significant: T effector memory: CD4+ CCR7- CD45RO+. *p≤0.05, **p≤0.01. Mean values with +/- SEM are shown. (a) – (b) Exact p-values (Supplementary Table 13) and n-numbers (Supplementary Table 14) were summarized in the supplementary tables.