Fig. 3.

Evaluation of the episignature detected for the combined cohort using hierarchical clustering and MDS. A Hierarchical clustering was applied, where rows and columns represented the selected probes and samples, respectively. The color scale indicated methylation levels from blue (no methylation or 0) to red (full methylation or 1). On the heatmap pane, red, orange, purple, and blue colors represented CHUJANS, BFLS, WHIKERS, and control samples, respectively. This plot demonstrates that the affected individuals’ samples can be clearly distinguished from controls using the selected set of probes. B The MDS plot, depicted using the same color scheme as the heatmap for sample types, clearly showed separation of the combined cohort from the control group