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. 2024 Jun 19;7:746. doi: 10.1038/s42003-024-06434-9

Fig. 1. Characterization of the interaction between hUHRF1 and hDPPA3.

Fig. 1

a Amino acid sequence alignment of C-terminal part of DPPA3. Secondary structures of mouse and human DPPA3 are indicated based on PDB:7XGA and analysis of this study, respectively. b Schematic of the domain composition of human UHRF1 and DPPA3. c Isothermal titration calorimetry measurements for hPHD and wild-type (WT)/mutants of hDPPA381-118. Superimposition of enthalpy change plots with standard deviations. Data were presented as mean values for n = 3. d Overlay of 1H-15N heteronuclear single quantum coherence (HSQC) spectra of 30 µM hPHD showing chemical shift changes upon titration with hDPPA381-118 of 0 µM (black), 15 µM (blue), 30 µM (green), and 60 µM (red). Square regions inside the HSQC spectra were expanded (lower panels).