Fig. 5. Functional assay of DPPA3 using Xenopus egg extracts.

a Experimental design for functional analysis of DPPA3 using Xenopus egg extracts. b Sperm chromatin was incubated with interphase Xenopus egg extracts supplemented with buffer (+buffer), 3×FLAG-mDPPA3, or 3×FLAG-hDPPA3. Chromatin fractions were isolated and immunoblotted using the indicated antibodies. The gel image is representative of n = 3 independent experiments. c Sperm chromatin was added to interphase egg extracts supplemented with radiolabeled S-[methyl-³H]-adenosyl-L-methionine and buffer (control), 3×FLAG-mDPPA3, or 3×FLAG-hDPPA3. The efficiency of DNA methylation maintenance was assessed by the incorporation of radio-labeled methyl groups from S-[methyl-³H]-adenosyl-L-methionine (³H-SAM) into DNA purified from the egg extracts. Data were presented as mean values ± SD for n = 3.