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Interaction of E2661 with recombinant CD81. Purified recombinant human CD81 LEL (h) and mouse CD81 LEL (m) proteins were analyzed by reducing (R) or nonreducing (NR) sodium dodecyl sulfate-polyacrylamide gel electrophoresis and detected with anti-GST MAb (A); anti-CD81 MAb 5A6 (B); E2661, followed by anti-E2 MAbs (C); and supernatant from mock-transfected cells, followed by anti-E2 MAbs (D). Numbers at the left indicate molecular mass, in kilodaltons.
