FIG. 6.

Affinity of E2 for cell surface-expressed CD81 variants. KM3 parental cells (solid graph) and transfectants expressing heterologous human CD81 (hCD81) and various mutants were tested for the ability to bind the anti-CD81 MAb 1.3.3.22 (5.0 μg/ml) (solid line) and a subsaturating concentration of E2₆₆₁ (0.5 μg/ml) (shaded line). Bound E2 was visualized with MAb H53 and anti-mouse IgG-PE. The results are from a single experiment, which are representative of comparable data obtained in two independent experiments.