TABLE 1.
Native but not reduced human CD81 LEL protein binds E2661, while anti-CD81 MAb 1D6 binds a defined reduced peptide within the LEL
Peptide | Human CD81 amino acids | DTTa | Binding of E2661 | Binding of 1D6 (μg/ml)
|
|||
---|---|---|---|---|---|---|---|
1.0 | 0.2 | 0.04 | 0.008 | ||||
1041 | 133–201 | + | 1.35 | 0.72 | 0.26 | 0.17 | |
1095 | 155–201 | + | 0.03 | 1.42 | 1.34 | 1.04 | 0.60 |
1101 | 164–201 | + | 0.21 | 1.45 | 1.30 | 0.87 | 0.41 |
1143 | 164–192 | + | 0.03 | 1.07 | 0.41 | 0.10 | 0.05 |
1144 | 173–192 | + | 0.02 | 1.25 | 0.48 | 0.30 | 0.05 |
1108 | 179–193 | + | 0.02 | 0.51 | 0.08 | 0.06 | 0.03 |
1077 | 146–155 | + | 0.03 | 0.05 | 0.05 | 0.02 | 0.03 |
1084 | 156–164 | + | 0.04 | 0.03 | 0.04 | 0.04 | 0.02 |
GST-human CD81 | LEL | − | 2.48 | 1.58 | 1.29 | 0.97 | 0.41 |
+ | 0.22 | 1.74 | 1.21 | 0.94 | 0.61 | ||
GST-mouse CD81 | LEL | − | 0.05 | 0.03 | 0.05 | 0.05 | 0.04 |
+ | 0.03 | 0.03 | 0.01 | 0.03 | 0.03 |
Peptides (10 mM) and proteins were incubated in PBS-dithiothreitol (DTT) (0.1 M) for 30 min at RT prior to coating of the ELISA plate.