TABLE 2.

Proliferation of T cells after coculture with splenocytes from adoptively reconstituted LCMV carrier mice

Bystander cellsa	103 Bystander cpm	Reconstituted carrier cellsb	103 Reconstituted cpm	103 Coculture cpmc
C57BL/6	121 ± 21	Anti-Thy + C′	3.0 ± .3	59 ± 4.1
C57BL/6	110 ± 16	Thy− (FACS)	3.5 ± .5	54 ± 5.7
C57BL/6	176 ± 6.3	Unseparated	2.2 ± .0	46 ± 0.5
C57BL/6	119 ± 18	Unseparated	4.7 ± .2	21 ± 0.9
B6 carrier	99 ± 13	Anti-Thy + C′	4.6 ± .6	112 ± 9.1
B6 carrier	55 ± 9.7	Thy− (FACS)	3.5 ± .5	43 ± 9.9
B6 carrier	169 ± 20	Unseparated	3.0 ± .3	34 ± 0.2
B6 carrier	232 ± 9.6	Unseparated	35 ± 4.7	96 ± 4.2

^aBystander cell spleen leukocytes from normal C57BL/6 or LCMV carrier C57BL/6 (B6 carrier) mice were placed into culture in the presence of anti-CD3 for 2 days and pulsed with [³H]thymidine, as described in Materials and Methods. 

^bSpleen leukocytes from LCMV-carrier mice that had been adoptively reconstituted for 6 days with splenocytes from LCMV-immune mice were isolated, processed as described in Materials and Methods, and either tested directly (unseparated) or after depletion of T cells with either monoclonal antibody to Thy 1.2 + complement (C′) or by staining with monoclonal antibody to Thy 1 and removal of the T cells by fluorescence activated cell sorting. These cells were examined for proliferation identically to the bystander cells. 

^cCocultured cells were 1:1 mixtures of the bystander and reconstituted carrier populations, dispensed at total cell concentrations equal to the totals of the separated cell populations. Thus, the cocultures have the same total number of cells as the individual bystander or reconstituted carrier cultures; consequently, there is a 1:2 dilution of the component cell numbers in the cocultures.