FIG. 3.

Induction of viral lytic proteins by Rta. S11E cells (10⁷) were electroporated with pcDNA3/MHV-68 rta (A) or pcDNA3 (B). Total protein from 10⁵ cells was collected at 12 (lanes 4 of panels A and B), 24 (lanes 5 of panels A and B), or 48 (lanes 6 of panels A and B) h posttransfection (p.t.). Proteins from 10⁴ uninfected (UI [lane 1]) or MHV-68-infected (I [lane 2]) BHK-21 cells or from 10⁵ untransfected S11E cells (UT [lanes 3]) were loaded and subjected to Western analysis with the rabbit hyperimmune serum against MHV-68-infected rabbit cell lysates. The molecular masses (kilodaltons) of individual proteins in the marker are indicated to the left.