FIG. 1.

Coupled in vitro transcription-translation of pcDNA3-based vaccine plasmids. All antigens encoded by the plasmids used for immunization of mice were expressed in vitro from the T7 promoter of the plasmid vector using the TnT (Promega) expression system. [³⁵S]methionine-labeled translation products were denatured in reducing SDS-polyacrylamide gel electrophoresis buffer, electrophoresed on gels of various acrylamide concentrations ranging from 5 to 15%, and visualized by fluorography or autoradiography. Each panel represents a different expression experiment and gel with the migration of the molecular mass standards (in kilodaltons) in each gel shown at the left.