KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-tyrosine hydroxylase | Millipore-Sigma | Cat#AB152; RRID:AB_390204 |
| Alexa-Fluor 647-conjugated donkey anti-rabbit | Invitrogen | Cat#A-32795; RRID:AB_2762835 |
| Guinea pig anti-GFAP | Synaptic Systems | Cat. # 173004; RRID:AB_10641162 |
| Rabbit anti-Iba1 | Wako Chemicals | Cat. # 019–19741; RRID:AB_839504 |
| Alexa594-conjugated goat anti-guinea pig | Life Technologies | Cat.#A-11076; RRID:AB_2534120 |
| Alexa488-conjugated goat anti-rabbit | Invitrogen | Cat. # A-11034; RRID:AB_2576217 |
| Donkey Serum | Sigma | Cat. # D9663 |
| Bacterial and virus strains | ||
| AAV1-hSyn-dLight1.3b | Lin Tian Lab | N/A |
| AAV9-CAG-dLight1.3b | Addgene | Cat#125560 |
| AAV5-hSyn-mCherry | Addgene | Cat#114472 |
| AAV1-syn-FLEX-jGCaMP8s-WPRE | Addgene | Cat#162377 |
| Chemicals, peptides, and recombinant proteins | ||
| α-methyl-4,5-dimethoxy-2-nitrobenzene oxymorphone (PhOX) | This paper | N/A |
| α-methyl-4,5-dimethoxy-2-nitrobenzene naloxone (PhNX) | This paper | N/A |
| Oxymorphone | Noramco | Cat#516340913 |
| Naloxone hydrochloride dihydrate | Sigma Aldrich | Cat#N7758 |
| Morphine sulfate | Spectrum | Cat#M1167 |
| DAMGO | Hello Bio | Cat#HB2409 |
| CTAP | Tocris | Cat#1560 |
| Critical commercial assays | ||
| GloSensor™ cAMP Assay | Promega | Cat#E1290 |
| NanoBiT® barrestin Recruitment Assays | Promega | Cat#N2014 |
| Deposited data | ||
| Source data | This paper | N/A |
| Experimental models: Cell lines | ||
| HEK 293T | ATCC | Cat#CRL-3216; RRID:CVCL_0063 |
| Experimental models: Organisms/strains | ||
| C57Bl/6 mice | Jackson Labs | Strain # 000664; RRID:IMSR_JAX:000664 |
| PvalbCre mice | Jackson Labs | Strain # 017320; RRID:IMSR_JAX:017320 |
| Rosa-Lsl-Td-tomato (Ai14) mice | Jackson Labs | Strain # 007914; RRID:IMSR_JAX:007914 |
| Slc32a1Cre mice (vGAT-cre) | Jackson Labs | Strain # 028862; RRID:IMSR_JAX:028862 |
| Long-Evans rats | Charles River | Strain Code: 006; RRID:RGD_2308852 |
| Recombinant DNA | ||
| pGloSensor −22F | Promega | Cat#E2301 |
| SSF-MOR (Mus musculus mu opioid receptor) | Banghart Lab and Williams Lab | Tanowitz and Von Zastrow26 |
| DOR (Mus musculus delta 1 opioid receptor) | Genscript | OMu22648D, Accession No. NM_013622.3 |
| KOR (Mus musculus kappa 1 opioid receptor) | cDNA Resource Center, www.cdna.org | Cat #OPRK100000 |
| β-arrestin2-LgBIT | Promega | N/A |
| hMOR-SmBIT | Promega | N/A |
| Software and algorithms | ||
| Matlab r2020a | MathWorks | RRID: SCR_001622 |
| Igor Pro 6 | Wavemetrics | RRID:SCR_000325 |
| GraphPad Prism 9 | Graphpad | RRID: SCR_002798 |
| pMAT | Barker Lab | Bruno etal.,202163 |
| Other | ||
| 375 nm laser | Vortran | Part#: 12084/B1 |
| 375 nm laser | Oxxius | Part#: LBX-375-HPE-PPA |