Figure 6.

Acinar specific ablation of Creb attenuates ADM reprogramming and increases tumor latency period with ACP induction in KC mice. (A) Representative H&E images of the pancreas along with co-immunofluorescent labeling (left) and quantification (right) of YFP (green)/CK19 (blue) in Ptf1a-^CreERTM/+;LSL-Kras^G12D/+;R26R-EYFP (KCY) and Creb^fl/fl (KCYC^−/−) as control or with ACP induction (n=4 mice per group). (B) qPCR-based analysis of pancreatic tissue harvested from KC and KCC^−/− mice with ACP induction for acinar and ductal genes (n=3 mice per group). (C) Quantitative assessment of tumor latency period in ctrl or with ACP induction in KC and KCC^−/− mice (n=12 mice per group). (D) Schematic representation illustrating that acinar-specific Creb ablation diminishes the progression of ADM/PanINs toward pancreatic cancer in the presence of ACP induction in KC mice. Scale bar, 50μm. ^nsnonsignificant; *p<0.05; ****p<0.0001 by ANOVA or unpaired t test.