Figure 4. HIV Env binding and neutralization assays of serum and IgG samples.

(a)-Neutralization assays were performed against twelve viruses from clades A, AC, AE, B, BC, C and G of tiers 2. The colors of the heatmap correspond to the IC₅₀ of the sera in micrograms per ml. The SIVmac251.30.SG3 virus is used as negative control. (b)-Antibody SOSIP interactions were determined by biolayer interferometry (BLI). The mAbs or bNAbs were loaded on a protein G biosensor, dipped into solution of the SOSIP trimer at different concentrations (ranging from 5 to 400 nM) and the nm shift was recorded. BLI sensorgrams are representative examples of experiments repeated two times. (c)-Neutralization assays were performed against twelve viruses from clades A, AC, AE, B, BC, C and G of tiers 2. The colors of the heatmap correspond to the IC₅₀ in micrograms per ml, for each antibody. The SIVmac251.30.SG3 virus is used as negative. (d)-Neutralization assays were performed against glycan mutated viruses to support epitope mapping to the CD4 binding site.