TABLE 2.
Primer sequences, repeat motifs, approximate fragment lengths, melting temperatures, GC content, number of observed alleles and polymorphic information content (PIC) in Abies grandis for 17 introduced polymorphic primer pairs.
| Primer | Primer sequence (5′‐3′) | Repeat motif | Fragment length | Tm (°C) | GC content (%) | Observed alleles | PIC |
|---|---|---|---|---|---|---|---|
| AGcp01‐F | TTCCAACCCCAAGTCTGGTC | C(12) | 202 | 59.52 | 55 | 4 | 0.200 |
| AGcp01‐R | GGATCGGAAATTGCATAAGCCTC | 60.06 | 48 | ||||
| AGcp02‐F | AAGTAGCTCCTGGATGGGGA | A(18) | 309 | 59.66 | 55 | 4 | 0.439 |
| AGcp02‐R | TTTCAACAAGTCGCACACCC | 59.26 | 50 | ||||
| AGcp03‐F | TCACACTGCTTTTCGGAGGG | T(17) | 124 | 60.25 | 55 | 2 | 0.699 |
| AGcp03‐F | TCGTGAAGCGAGAAAGGTGT | 59.61 | 50 | ||||
| AGcp04‐F | TCATTGGGTTCYTTGGRCCTT | G(12) | 214 | 60.13 | 48 | 3 | 0.207 |
| AGcp04‐R | TTGCCTCTCCTGATGGTTGG | 59.67 | 55 | ||||
| AGcp05‐F | TCGATCCATTTCCACCGGTAT | T(15) | 154 | 58.96 | 48 | 5 | 0.419 |
| AGcp05‐R | TCCGATCTGAATTACGGAAACCT | 59.55 | 43 | ||||
| AGcp06‐F | TGGACCATGAAAATGAAAGAATGGA | A(16) | 205 | 59.22 | 36 | 5 | 0.634 |
| AGcp06‐R | TGGGTAAGTCTTAGGACCCG | 58.14 | 55 | ||||
| AGcp09‐F | ACCTCAGCTATGTCCCTCGT | A(11) | 201 | 60.03 | 55 | 3 | 0.338 |
| AGcp09‐R | CGATCGGTCGCCAGGATAAA | 59.97 | 55 | ||||
| AGcp11‐F | GGGAAGAAAGAACATTTGGAAAACA | T(16) | 205 | 58.65 | 36 | 6 | 0.674 |
| AGcp11‐R | ACGTAATCTCCGGGATCCTTATT | 58.84 | 43 | ||||
| AGcp15‐F | ACCATTCAACCATACCCGCA | AT(7) | 173 | 59.67 | 50 | 3 | 0.223 |
| AGcp15‐R | AATGAAGGTGCTCAAGGGAGG | 59.99 | 52 | ||||
| AGcp18‐F | TTGGTACGGCACTTGAGAGA | T(17) | 169 | 58.67 | 50 | 7 | 0.510 |
| AGcp18‐R | AGTGACATCAATAACTGGTCCAA | 57.76 | 39 | ||||
| AGcp19‐F | CATGCCAACCACTCAACTCAC | A(13)T(5) | 230 | 59.73 | 52 | 3 | 0.201 |
| AGcp19‐R | TGACGTGGTGGAAGTCATCAG | 60 | 52 | ||||
| AGcp20‐F | GTGTTCCTCTATCCGTGGAGT | T(20) | 207 | 58.9 | 52 | 8 | 0.569 |
| AGcp20‐R | GAGGCGTACATCTCTTCTGGT | 59.25 | 52 | ||||
| AGcp21‐F | GCTTACCCTACATGGTCGAGA | A(19) | 123 | 58.97 | 52 | 8 | 0.803 |
| AGcp21‐R | TTCCTGGTATTGTCCAAGAATAGT | 57.36 | 38 | ||||
| AGcp22‐F | CTGCTGGATGCAGAGGAACT | T(14) | 161 | 59.75 | 55 | 3 | 0.493 |
| AGcp22‐R | TCCGATGGATTGTTACTGTGTATTG | 59.18 | 40 | ||||
| AGcp23‐F | TGGATTCGGTCCATTGATTGC | T(9)‐A‐C(9)T(7) | 178 | 58.98 | 48 | 8 | 0.780 |
| AGcp23‐R | CCATATTAGTTGACACRAKMTTTCA | 57.23 | 36 | ||||
| AGcp24‐F | GACCGATCATTGCGGGTACA | T(16) | 334 | 60.18 | 55 | 6 | 0.510 |
| AGcp24‐R | ATCCTCATGGAGGTGGGGAA | 59.95 | 55 | ||||
| AGcp25‐F | ACCCTTTTCCGAGGGGTAGT | T(23) | 351 | 60.18 | 55 | 10 | 0.773 |
| AGcp25‐R | ACCTCATACGGCTCCTCCTT | 60.03 | 55 |