TABLE A2.

Information about the initially non‐polymorphic chloroplast SSR markers, developed for Abies grandis.

Primer	Primer sequence (5′‐3′)	Pepeat motif	Fragment length	Tm (°C)	GC content (%)
AGcp07‐F	TACCATCCCCATCAGAACGA	T(13)	259	57.83	50
AGcp07‐R	GACGATCATAGGTCGGGAGT			58.39	55
AGcp08‐F	ATCGATATCAATACTCCAATACGCT	TA(8)	149	57.9	36
AGcp08‐R	TTTGAGTCTCGCGTGTCTACC			60.07	52
AGcp10‐F	GTYGCACGTTGCTTTCTACC	T(5)	70	59.84	55
AGcp10‐R	TGATTCGAGATCAGSAGGGAG			59.31	52
AGcp12‐F	TTCTTCCGAGAACCACCCAA	C(4)	177	58.87	50
AGcp12‐R	TGGGCAACCCTCTGAACAAC			60.47	55
AGcp13‐F	ATGGTCCTCGGATTTCTCCT	A(5)	241	57.82	50
AGcp13‐R	TGACTGATGCTTTTCCACCCA			59.86	48
AGcp14‐F	CGGGCTCCACTGTTATCCG	A(14)	436	60.23	63
AGcp14‐R	GCAGATAGAAGCCGGACGAA			59.9	55
AGcp16‐F	TGAAGCGAATGGAGACAAGTTA	C(8)	124	57.73	41
AGcp16‐R	TCCCTACCCTCTCGTGTCAA			59.59	55
AGcp17‐F	TGGAGACTGGGAATTRACGG	T(16)	232	59.39	55
AGcp17‐R	GCACGGATAGAAGTGGGTGA			59.46	55