VSVG, B5R, and GFP chimeras. (A) Schematic representation of chimeras. VSVG-GFP and B5R-GFP are VSVG and B5R proteins with GFP appended to the cytoplasmic tail, respectively. Chimeras are constructed from the lumenal domain (LD), transmembrane domain (TMD), and cytoplasmic tail (CT) of VSVG (■) or B5R (□), and GFP (). ▵, deleted cytoplasmic domain. (B) Amino acid sequences of predicted transmembrane domains (underlined) and cytoplasmic tails of B5R and VSVG. Tyrosine 310 and leucines 315 and 316 of B5R and the diacidic signal of VSVG are shown in italics.