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Localization of chimeric proteins following a chase in the presence of cycloheximide. COS-7 cells expressing the indicated GFP chimeric proteins were incubated in medium containing 100 μg of cycloheximide per ml at 31°C. Coverslips were removed at 0, 4, and 8 h after addition of cycloheximide and stained with Hoechst dye. Colors: green, GFP; blue, Hoechst dye.
