FIG. 1.

Constitutively active IRF-3 is toxic to cells. 293 cells were transfected with 10 μg of control pEGFPC1 vector (A), pEGFPC1-wtIRF-3 (B), or pEGFPC1-IRF-3(5D) (C). Beginning 36 h after transfection, cells were selected in media containing G418 (400 μg/ml); after 2 weeks of selection, cells were fixed in the plate with ice-cold methanol and were stained with Giemsa. Expression of the GFP fusion proteins was monitored by fluorescence microscopy.