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. 2024 Jun 18;16(1):2365891. doi: 10.1080/19420862.2024.2365891

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© 2024 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 1. — Integrin specificity of antibodies on all RGD-binding human and mouse integrin transfectants by indirect flow cytometry. (a) K562 stable human integrin transfectants in Ca²⁺/Mg²⁺. (b) Expi293 α5⁻/αV⁻ cell transient human integrin transfectants in Ca²⁺/Mg²⁺. (c-g) Expi293 α5⁻/αV⁻ cell transient mouse integrin transfectants in Ca²⁺/Mn²⁺. Immunostaining was performed with 50 nM IPI integrin antibody followed by washing and detection with APC-conjugated goat anti-human secondary antibodies and flow cytometry. MFI: mean fluorescence intensity.