Figure 2.

Titration of antibodies on human RGD-binding integrin K562 stable transfectants by indirect flow cytometry. All antibodies were titrated against each transfectant in Ca²⁺/Mg²⁺ and immunostaining was as in Figure 1. The mean fluorescent intensity (MFI) at each antibody concentration after subtraction of isotype control at the same concentration was fitted to a three-parameter dose–response curve for EC₅₀, background MFI, and maximum MFI; curves are only shown for antibodies with meaningful staining. The errors for the EC₅₀ values are the standard errors from the non-linear least square fits.