Figure 3.

Surface plasmon resonance (SPR) binding kinetics with soluble integrin ectodomains. (a-m). Antibodies were captured on the surface with anti-Fc. Integrins in 10 mM HEPES pH 7.5, 150 mM NaCl, 1 mM MgCl₂, 1 mM CaCl₂, 0.05% Tween 20, and 0.5 mg/mL BSA were used at 0.78, 1.56, 3.12, 6.25, and 12.50 nM. SPR sensorgrams (thick gray lines) at each ectodomain concentration were globally fitted with 1 vs 1 Langmuir binding model for the on- and off-rates, k_on and k_off. K_D values were calculated as k_off/k_on. Values are reported as means with standard deviations from three independent regions of interest (ROIs).