Fig. 1. PAGE analysis of in situ nicked-duplex RNA ligation using 5′-pUACUGGCA-Cy3 (10 μM), 5′-GAGAACC (20 μM), 5′-CCAGUAGGUUCUC (10 μM), 1-MeIm (100 mM), KCN (50 mM), sodium hypochlorite (20 mM), pH value was 6, (a) before sodium hypochlorite was added; (b) with MgCl2 (10 mM), incubated for 2 hours; (c) with MnCl2 (10 mM), incubated for 2 hours; (d) same as b, incubated for 24 hours; (e) same as c, incubated for 24 hours.
