FIG. 4.

Recovery of infectious HSV from G207-challenged brains. Whole brain homogenates, from surviving KOS i.c.-inoculated BALB/c mice that were rechallenged with G207 (10⁷ PFU) on day 0, were titered on Vero cells, and the PFU per brain was determined. Plaques that stained with X-Gal were considered G207, and those that did not were considered KOS. No plaques were detected from day 4 onward; no white plaques were found at any time point. The minimal detectable titer was >30 PFU per brain. Two animals were sacrificed at each time point.