TABLE 1.
Primer pairs used in this study
Namea | Gene | Primer sequenceb | PCR product size (bp) | Reference |
---|---|---|---|---|
RR (ICP6) | UL39 | 5′ GACAG CCATA TCCTG AGC 3′ | 221 | 15 |
3′ GCCAG CAGTT GCTAG ACACT CA 5′ | ||||
TK | UL23 | 5′ CTGCA GATAC CGCTC CGTAT T 3′ | 273 | 46 |
3′ TACCC TACCG CCAGC TTCTA C 5′ | ||||
gE | US8 | 5′ GAGATGCGAATATACGAAT 3′ | 320 | 18 |
3′ TAAAC CGGCT CGGGT GGGTG 5′ | ||||
LacZ | 5′ TTGCT GATTC GAGGG GTTAA CCGTC ACGAG 3′ | 300 | 64 | |
3′ TAGCA TTAGT GGCGT CACAC TAGTAG ACCA 5′ | ||||
Fx | 5′ AGACT CTTAC CTTTG GGGAT A 3′ | 127 | 40 | |
3′ TCGAT TACTT CGTGA CCAGT G 5′ |
Fx is a single-copy mouse gene, RR, TK, and gE amplify HSV-1 sequences, and LacZ is unique to G207.
Sequences listed 5′ to 3′ are upstream primers; 3′-to-5′ sequences are downstream primers.