TABLE 4.
Detection of viral sequences in the brain by PCRa
| Injection
|
Primer
|
No. of animals
|
||||||
|---|---|---|---|---|---|---|---|---|
| 1st | 2nd | RR | TK | gE | LacZ | Fx | PCR | Alive |
| KOS | VB | + | + | + | − | + | 1 | 3 |
| G207 | + | + | + | + | + | 3 | 5 | |
| VB | VB | − | − | − | − | + | 2 | 3 |
| G207 | + | + | + | + | + | 2 | 3 | |
a
Three-week-old BALB/c female mice were i.c. inoculated with ≤2 × 103 PFU of KOS or VB (first injection); after viral latency had been established (8 to 9 weeks), G207 (107 PFU) or VB was inoculated at the same stereotactic site (second injection). Animals were sacrificed 8 weeks after the second injection, except in the KOS/G207 group, where an animal was sacrificed at 8, 16, and 24 weeks (PCR); DNA was extracted from brain tissue and subjected to PCR with the indicated primers (Table 1). The remainder of the animals were alive and well at 1.7 years.