A, Maximum intensity projection of a 2p microtomo-graphic image z-stack of BerEP4-negative (UACC257 melanoma) microtumor phantom lacking signal from cells on microsphere labeled with anti-BerEP4(594). The microbead is seen because of nonspecific binding of fluorescent Ab to the collagen on the surface of the microsphere. B, Backscatter mode z-stack projection of the same phantom shown on A confirming the presence of UACC257cells on microbeads, once again confirming the negative predictive value of BerEP4 Ab as a molecular imaging probe. C, Tomographic image (section 50 at 0.5-micron section tomography) of A. D, Tomographic image (section 50 at 0.5-micron section tomography) of B. Scale bar 5 100 microns.