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Effect of dose on AAV2/βGal- and AAV5/βGal-mediated gene transfer to human airway epithelia. Human airway epithelia were exposed to increasing concentrations of AAV2/βGal and AAV5/βGal from the apical surface. The epithelia were then rinsed after 1 h and were incubated for 2 weeks prior to analysis of β-galactosidase activity. Data are β-galactosidase activities per mg of protein ± SEMs (n = 4).
