FIG. 5.

Effect of soluble heparin on AAV gene transfer to human airway epithelia. To compete off AAV binding and gene transfer, in some conditions the viruses were pretreated with 20 μg of soluble heparin per ml for 30 min. The figure shows the effect of heparin on AAV gene transfer to human airway epithelia from the apical side (A) and from the basolateral side (B). Five hundred particles per cell of either AAV2/βGal or AAV5/βGal were added for 30 min at 4°C. β-Galactosidase activity was measured 14 days later. Data are means ± SEMs (n = 8 in each group). Asterisk indicates P = 0.018.