Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Mar 8;31(6):925–938. doi: 10.1038/s41594-024-01246-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 4 — a, AF2 model of DENND10 region of the Commander complex used as an initial model with predicted alignment error plots indicating the relevant chains. Model is colored according to per-residue pLDDt scores. Model has been trimmed based on the fit to the cryo-EM density map. AF2 model contained all chains of the bottom half during prediction. b, V-coil region of Commander as in a, with different random seed in AF2 prediction. c, Retriever subcomplex model as in a. d, Chemical crosslinks identified by MS in the context of the Commander structure model. e, Comparison of DENND1B-Rab35 complex structure (PDB 3TW8) with DENND10 in the context of Commander. I-coil sterically blocks the putative Rab binding site on DENND10. f, Structure of Retriever in the context of the Commander complex compared to the Fungal retromer structure. Interface 1: VPS29-VPS35(L). Interface 2: VPS35(L)-VPS26(C). Retriever adopts a contracted conformation compared to retromer and exhibits larger interaction interfaces. g, Comparison of Commander complex models presented in this study (left) and in Healy et al. (right), kindly provided by the authors, superposed via DENND10. h, Density supporting the placement of CCDC22 α15 and α16. Map (EMD-17340) was low-pass filtered to 7 Å.