Fig. 5. Analysis of overall tertiary fold of the endogenous Commander complex compared to existing literature.

a, A simplified ‘ragdoll’ representation of major components of the Commander complex from this study. b–d, The overall structural model from Healy et al.⁵⁷ highlight the major differences between these models with alignment centers of the models located at the COMMD-ring (b), V-coil (c) and DENND10 (d). The COMMD-ring is represented by a disc aligned to the COMMD domains, the coiled-coil domains are represented by cylinders (I- and R-coils) or a trapezoidal prism (V-coil + CCDC22 NN–CH), DENND10 as a cylinder and Retriever subcomplex as spheres (VPS29, VPS26C + N-terminal half of VPS35L α-solenoid) or a cylinder (VPS35L C-terminal half of VPS35L α-solenoid). Component relative rotation angles are calculated based on the underlying atomic coordinates of backbone Cα atoms.