Fig. 2. MatAbs against SARS-CoV-2 are transferred to mouse pups and wane over time.

a The experimental design is shown; Female mice were mated with males and then were inoculated at day 5 after introduction of males with SARS-CoV-2 mRNA-LNP vaccine at 0.1 µg, 1 µg, or 5 µg. b, c. b Sera were collected from mouse pups at indicated time points after weaning/vaccination and SARS-CoV-2 full length Spike (S) protein-specific IgG concentrations were measured by ELISA. One phase-decay was fitted to IgG data (R² > 0.90 for all groups) with each point representing a single mouse pup and each line representing the decay curve for one dose group (0.1 µg n = 2; 1 µg n = 12; 5 µg n = 7). c Dams were vaccinated with 5 µg mRNA-LNP and serum was collected from dams (n = 6) and pups (n = 48) at day of weaning. Each point represents the geometric mean with error bars indicating 95% confidence interval (CI) of the geometric mean. Sample IgG concentrations were plotted to a standard curve of known concentrations of a S-specific IgG monoclonal antibody and are reported as arbitrary units (AU)/mL. Data are shown as geometric mean concentrations with 95% confidence intervals (* indicates p < 0.05 by unpaired two-tailed Student’s T test). Schematic for Figure a created with Biorender.