Fig. 3. RNA velocity analysis indicates that TBI induces a subtle but functionally significant shift in hippocampal neural stem cell fate.
RNA velocity analysis along both the neuronal and astrocytic lineage in the Control (a) and TBI (b) groups, vectors (arrows) indicate the predicted direction and speed of single-cell transitions in transcriptome space, colors indicate previously characterized cell clusters indicated in the figures. Box blots showing transition probabilities calculated for NSC-stage 1 cells to NSC-stage 1 cells (****P = 4.4 × 10−15), to NSC-stage 2 (****P = 3.7 × 10−15) and to RG-like cells (**P = 1.5 × 10−3) (c); NSC-stage 2 cells to NSC-stage 1 (****P = 6.5 × 10−8), to NSC-stage 2 (*P = 1.7 × 10−2), to RG-like (****P = 1.4 × 10−5) and to N-stage 1 cells (****P = 7.9 × 10−5) (d); RG-like cells to NCS-stage 1 (**P = 1.2 × 10−3), to RG-like (****P = 6.6 × 10−12), to N-stage 1 (****P = 9.3 × 10−4) and to A-stage 1 cells (****P = 3.6 × 10−6) (e); N-stage 1 cells to RG-like (*P = 4.7 × 10−2), to N-stage 1 (***P = 7.1 × 10−4), and to N-stage 2 cells (**P = 1.7 × 10−3) (f); and A-stage 1 cells to A-stage 1 (****P = 5.4 × 10−9) and to A-stage 2 cells (****P = 3.9 × 10−9) (g). Orange bars: Control group, cyan bars: TBI group, using Control = 5 and TBI = 6 animals per condition. In all panels, independent non-parametric two-sided Wilcoxon test, Control vs TBI. Box plots show the median, first quartile (25%), third quartile (75%), and interquartile range. Whiskers represent data minima and maxima; dots are data points located outside the whiskers, n = number of cells per cell subpopulation Control/TBI are available as Supplementary Data 2 and calculated mean transition probabilities across cell clusters are available in Supplementary Data 14, including exact P values for all comparisons. Source data are provided as Supplementary Data 15.